RBD#4L
(Plasmid
#193021)
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PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepC013-Twinstrep-SUMO-huLwCas13a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas13a
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SpeciesLeptotrichia wadei
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Tag
/ Fusion Protein
- hnRNP C RRM
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RBD#4L was a gift from Xue Gao (Addgene plasmid # 193021 ; http://n2t.net/addgene:193021 ; RRID:Addgene_193021) -
For your References section:
Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection. Yang J, Song Y, Deng X, Vanegas JA, You Z, Zhang Y, Weng Z, Avery L, Dieckhaus KD, Peddi A, Gao Y, Zhang Y, Gao X. Nat Chem Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 10.1038/s41589-022-01135-y. 10.1038/s41589-022-01135-y PubMed 36138140