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Addgene

RBD#3L
(Plasmid #193020)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193020 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pC013-Twinstrep-SUMO-huLwCas13a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas13a
  • Species
    Leptotrichia wadei
  • Tag / Fusion Protein
    • hnRNP A1 RRM2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RBD#3L was a gift from Xue Gao (Addgene plasmid # 193020 ; http://n2t.net/addgene:193020 ; RRID:Addgene_193020)
  • For your References section:

    Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection. Yang J, Song Y, Deng X, Vanegas JA, You Z, Zhang Y, Weng Z, Avery L, Dieckhaus KD, Peddi A, Gao Y, Zhang Y, Gao X. Nat Chem Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 10.1038/s41589-022-01135-y. 10.1038/s41589-022-01135-y PubMed 36138140