AAV-hSYN-TdTomato
(Plasmid
#193019)
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PurposeAAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAddgene plasmid #51506
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Backbone manufacturerHongkui Zeng
- Total vector size (bp) 5145
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Modifications to backboneAAV-hSYN-TdTomato was made by removing the WPRE from Addgene plasmid #51506 by ClaI digestion
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTdTomato
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Insert Size (bp)1431
- Promoter hSYN
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer not applicable
- 3′ sequencing primer not applicable (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-hSYN-TdTomato was a gift from Bart Nieuwenhuis (Addgene plasmid # 193019 ; http://n2t.net/addgene:193019 ; RRID:Addgene_193019)
Map uploaded by the depositor.
