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Addgene

pSLED.CaRPv1
(Plasmid #193018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
  • Backbone manufacturer
    Addgene
  • Modifications to backbone
    Modified entire sequence between AAV2 ITRs
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)
  • Alt name
    jGCaMP7b
  • Alt name
    jRGECO1a
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLED.CaRPv1 was a gift from Jonathan Ling (Addgene plasmid # 193018 ; http://n2t.net/addgene:193018 ; RRID:Addgene_193018)

  • For your References section:

    Cell-specific regulation of gene expression using splicing-dependent frameshifting. Ling JP, Bygrave AM, Santiago CP, Carmen-Orozco RP, Trinh VT, Yu M, Li Y, Liu Y, Bowden KD, Duncan LH, Han J, Taneja K, Dongmo R, Babola TA, Parker P, Jiang L, Leavey PJ, Smith JJ, Vistein R, Gimmen MY, Dubner B, Helmenstine E, Teodorescu P, Karantanos T, Ghiaur G, Kanold PO, Bergles D, Langmead B, Sun S, Nielsen KJ, Peachey N, Singh MS, Dalton WB, Rajaii F, Huganir RL, Blackshaw S. Nat Commun. 2022 Oct 1;13(1):5773. doi: 10.1038/s41467-022-33523-2. 10.1038/s41467-022-33523-2 PubMed 36182931
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