pSLED.GluA2
(Plasmid
#193017)
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PurposeGluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
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Backbone manufacturerAddgene
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Modifications to backboneModified entire sequence between AAV2 ITRs
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBichromatic reporter (EGFP and dsRed-Express2)
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Alt nameEGFP
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Alt nameDsRed
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLED.GluA2 was a gift from Jonathan Ling (Addgene plasmid # 193017 ; http://n2t.net/addgene:193017 ; RRID:Addgene_193017) -
For your References section:
Cell-specific regulation of gene expression using splicing-dependent frameshifting. Ling JP, Bygrave AM, Santiago CP, Carmen-Orozco RP, Trinh VT, Yu M, Li Y, Liu Y, Bowden KD, Duncan LH, Han J, Taneja K, Dongmo R, Babola TA, Parker P, Jiang L, Leavey PJ, Smith JJ, Vistein R, Gimmen MY, Dubner B, Helmenstine E, Teodorescu P, Karantanos T, Ghiaur G, Kanold PO, Bergles D, Langmead B, Sun S, Nielsen KJ, Peachey N, Singh MS, Dalton WB, Rajaii F, Huganir RL, Blackshaw S. Nat Commun. 2022 Oct 1;13(1):5773. doi: 10.1038/s41467-022-33523-2. 10.1038/s41467-022-33523-2 PubMed 36182931