Zic4-3505-D2:Luc
(Plasmid
#193004)
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PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D2)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHydra Zic4 promoter
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SpeciesHydra
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Insert Size (bp)2493
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Mutationpartial deletion of the Hydra Zic4 promoter-D2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit for https://www.biorxiv.org/content/10.1101/2021.12.22.473838v1 bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zic4-3505-D2:Luc was a gift from Brigitte Galliot (Addgene plasmid # 193004 ; http://n2t.net/addgene:193004 ; RRID:Addgene_193004) -
For your References section:
The transcription factor Zic4 acts as a transdifferentiation switch. Vogg MC, Ferenc J, Buzgariu WC, Perruchoud C , Papasaikas P, Sanchez PGL, Nuninger C , Delucinge-Vivier C , Rampon C , Beccari L, Vriz S , Vincent S , Galliot B , Tsiairis CD. bioRxiv 2021.12.22.47383 10.1101/2021.12.22.473838