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Addgene

Zic4-3505:GFP
(Plasmid #193001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193001 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HyActin-RFP::HyWnt3FL-EGFP
  • Vector type
    Hydra transgenesis vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hydra Zic4 promoter
  • Species
    Hydra
  • Insert Size (bp)
    3505
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zic4-3505:GFP was a gift from Brigitte Galliot (Addgene plasmid # 193001 ; http://n2t.net/addgene:193001 ; RRID:Addgene_193001)

  • For your References section:

    The transcription factor Zic4 acts as a transdifferentiation switch. Vogg MC, Ferenc J, Buzgariu WC, Perruchoud C , Papasaikas P, Sanchez PGL, Nuninger C , Delucinge-Vivier C , Rampon C , Beccari L, Vriz S , Vincent S , Galliot B , Tsiairis CD. bioRxiv 2021.12.22.47383 10.1101/2021.12.22.473838
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