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Addgene

ZfSp5a-377
(Plasmid #192997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zebrafish Sp5
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1131
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a S357F mutation in Zebrafish Sp5-HA. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZfSp5a-377 was a gift from Brigitte Galliot (Addgene plasmid # 192997 ; http://n2t.net/addgene:192997 ; RRID:Addgene_192997)

  • For your References section:

    An evolutionarily-conserved Wnt3/beta-catenin/Sp5 feedback loop restricts head organizer activity in Hydra. Vogg MC, Beccari L, Iglesias Olle L, Rampon C, Vriz S, Perruchoud C, Wenger Y, Galliot B. Nat Commun. 2019 Jan 18;10(1):312. doi: 10.1038/s41467-018-08242-2. 10.1038/s41467-018-08242-2 PubMed 30659200
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