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HyWnt3-ΔRep-D2:Luc
(Plasmid #192989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hydra Wnt3 promoter
  • Species
    Hydra
  • Insert Size (bp)
    1864
  • Mutation
    partial deletion of the Wnt3 repressor element-D2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HyWnt3-ΔRep-D2:Luc was a gift from Brigitte Galliot (Addgene plasmid # 192989 ; http://n2t.net/addgene:192989 ; RRID:Addgene_192989)

  • For your References section:

    An evolutionarily-conserved Wnt3/beta-catenin/Sp5 feedback loop restricts head organizer activity in Hydra. Vogg MC, Beccari L, Iglesias Olle L, Rampon C, Vriz S, Perruchoud C, Wenger Y, Galliot B. Nat Commun. 2019 Jan 18;10(1):312. doi: 10.1038/s41467-018-08242-2. 10.1038/s41467-018-08242-2 PubMed 30659200
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