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Addgene

pGL2-eNOSPromoter-Luciferase
(Plasmid #19297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19297 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    endothelial nitric oxide synthase promoter
  • Alt name
    eNOS
  • Alt name
    NOS3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1621
  • GenBank ID
    L23210
  • Entrez Gene
    NOS3 (a.k.a. ECNOS, eNOS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-eNOSPromoter-Luciferase was a gift from Charles Lowenstein (Addgene plasmid # 19297 ; http://n2t.net/addgene:19297 ; RRID:Addgene_19297)

  • For your References section:

    Regulation of endothelial nitric-oxide synthase during hypoxia. Arnet UA, McMillan A, Dinerman JL, Ballermann B, Lowenstein CJ. J Biol Chem. 1996 Jun 21. 271(25):15069-73. 10.1074/jbc.271.25.15069 PubMed 8663208
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