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Addgene

pET29a(+)::D1141A
(Plasmid #192963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tse5_D1141A
  • Species
    Pseudomonas aeruginosa
  • Mutation
    D1141A
  • Tag / Fusion Protein
    • 9xhis + TEV tag (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29a(+)::D1141A was a gift from David Albesa-Jové (Addgene plasmid # 192963 ; http://n2t.net/addgene:192963 ; RRID:Addgene_192963)

  • For your References section:

    The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria. Gonzalez-Magana A, Altuna J, Queralt-Martin M, Largo E, Velazquez C, Montanchez I, Bernal P, Alcaraz A, Albesa-Jove D. Commun Biol. 2022 Nov 5;5(1):1189. doi: 10.1038/s42003-022-04140-y. 10.1038/s42003-022-04140-y PubMed 36335275
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