pBS-iNOS
(Plasmid #19295)

• Depositing Lab: Charles Lowenstein
• Publication: Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89(15):6711-5.

Backbone

• Vector backbone: pBluescript KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: inducible nitric oxide synthase
• Alt name: iNOS
• Alt name: NOS2
• Species: M. musculus (mouse)
• Insert Size (bp): 4110
• GenBank ID: M92649 NP_035057.1
• Entrez Gene: Nos2 (a.k.a. MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry