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pAAV-OTp-GCaMP6s
(Plasmid #192945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 7991
  • Modifications to backbone
    n/a
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    n/a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6s
  • Species
    R. norvegicus (rat), G. gallus (chicken); Jellyfish
  • Insert Size (bp)
    1353

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CACCATCGCCGAATTCatgggttctcatcatcatca
  • 3′ sequencing primer ggccgcatatAAGCTTtcacttcgctgtcatcattt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-OTp-GCaMP6s was a gift from Kazunari Miyamichi (Addgene plasmid # 192945 ; http://n2t.net/addgene:192945 ; RRID:Addgene_192945)

  • For your References section:

    Dynamic modulation of pulsatile activities of oxytocin neurons in lactating wild-type mice. Yaguchi K, Hagihara M, Konno A, Hirai H, Yukinaga H, Miyamichi K. PLoS One. 2023 May 10;18(5):e0285589. doi: 10.1371/journal.pone.0285589. eCollection 2023. 10.1371/journal.pone.0285589 PubMed 37163565
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