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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
(Plasmid #192939)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192939 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAVS1
  • Total vector size (bp) 15397
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    AP1(2)/minTK promoter
  • Insert Size (bp)
    142

Gene/Insert 2

  • Gene/Insert name
    FlpO-ABI
  • Insert Size (bp)
    2166

Gene/Insert 3

  • Gene/Insert name
    PYL-FlpO
  • Insert Size (bp)
    708

Gene/Insert 4

  • Gene/Insert name
    CAG promoter
  • Insert Size (bp)
    1701

Gene/Insert 5

  • Gene/Insert name
    frt-STOP-frt
  • Insert Size (bp)
    1227

Gene/Insert 6

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    717

Gene/Insert 7

  • Gene/Insert name
    luciferase
  • Insert Size (bp)
    1653

Gene/Insert 8

  • Gene/Insert name
    pPGK
  • Insert Size (bp)
    504

Gene/Insert 9

  • Gene/Insert name
    Zeocin resistance
  • Insert Size (bp)
    372

Gene/Insert 10

  • Gene/Insert name
    BFP
  • Insert Size (bp)
    696

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    AP1(2) short response element sequence is from Affymetrix.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR was a gift from Wilson Wong (Addgene plasmid # 192939 ; http://n2t.net/addgene:192939 ; RRID:Addgene_192939)
  • For your References section:

    Engineering digitizer circuits for chemical and genetic screens in human cells. Wong NM, Frias E, Sigoillot FD, Letendre JH, Hild M, Wong WW. Nat Commun. 2021 Oct 22;12(1):6150. doi: 10.1038/s41467-021-26359-9. 10.1038/s41467-021-26359-9 PubMed 34686672