pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
(Plasmid
#192939)
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PurposeFlpO-based digitizer circuit driven by AP1 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAVS1
- Total vector size (bp) 15397
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameAP1(2)/minTK promoter
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Insert Size (bp)142
Gene/Insert 2
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Gene/Insert nameFlpO-ABI
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Insert Size (bp)2166
Gene/Insert 3
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Gene/Insert namePYL-FlpO
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Insert Size (bp)708
Gene/Insert 4
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Gene/Insert nameCAG promoter
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Insert Size (bp)1701
Gene/Insert 5
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Gene/Insert namefrt-STOP-frt
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Insert Size (bp)1227
Gene/Insert 6
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Gene/Insert nameGFP
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Insert Size (bp)717
Gene/Insert 7
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Gene/Insert nameluciferase
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Insert Size (bp)1653
Gene/Insert 8
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Gene/Insert namepPGK
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Insert Size (bp)504
Gene/Insert 9
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Gene/Insert nameZeocin resistance
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Insert Size (bp)372
Gene/Insert 10
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Gene/Insert nameBFP
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Insert Size (bp)696
Resource Information
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A portion of this plasmid was derived from a plasmid made byAP1(2) short response element sequence is from Affymetrix.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR was a gift from Wilson Wong (Addgene plasmid # 192939 ; http://n2t.net/addgene:192939 ; RRID:Addgene_192939) -
For your References section:
Engineering digitizer circuits for chemical and genetic screens in human cells. Wong NM, Frias E, Sigoillot FD, Letendre JH, Hild M, Wong WW. Nat Commun. 2021 Oct 22;12(1):6150. doi: 10.1038/s41467-021-26359-9. 10.1038/s41467-021-26359-9 PubMed 34686672