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Addgene

pBabePuro-hSNAIL.ER.NoTag
(Plasmid #19292)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabePuro
  • Backbone manufacturer
    Alan Friedman
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNAIL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    810
  • Entrez Gene
    SNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
  • Tag / Fusion Protein
    • mouse ERalpha ligand-binding domain (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE-5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

No epitope tag - can be detected on western blot using alpha-ER Ab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabePuro-hSNAIL.ER.NoTag was a gift from Eric Fearon (Addgene plasmid # 19292 ; http://n2t.net/addgene:19292 ; RRID:Addgene_19292)

  • For your References section:

    The SLUG zinc-finger protein represses E-cadherin in breast cancer. Hajra KM, Chen DY, Fearon ER. Cancer Res. 2002 Mar 15. 62(6):1613-8. PubMed 11912130
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