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Addgene

pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
(Plasmid #19291)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19291 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5568
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-cadherin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    233
  • Mutation
    E-cad promoter sequences from -108 to +125 Ebox elements were mutated from 5'-CANNTG-3' to 5'-AANNTA-3' (sense strand)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer luc-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)

33 bp linker between Ecad promoter and luciferace gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT was a gift from Eric Fearon (Addgene plasmid # 19291 ; http://n2t.net/addgene:19291 ; RRID:Addgene_19291)

  • For your References section:

    The SLUG zinc-finger protein represses E-cadherin in breast cancer. Hajra KM, Chen DY, Fearon ER. Cancer Res. 2002 Mar 15. 62(6):1613-8. PubMed 11912130
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