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pX330-FOXL2-cterm
(Plasmid #192893)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192893 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 42230)
  • Backbone size w/o insert (bp) 8466
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXL2 C-term sgRNA
  • gRNA/shRNA sequence
    CGTCTGCACCGGCATGCGGT
  • Species
    Synthetic
  • Insert Size (bp)
    21
  • Entrez Gene
    FOXL2 (a.k.a. BPES, BPES1, PFRK, PINTO, POF3)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also expresses Cas9 with nuclear localization signals.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-FOXL2-cterm was a gift from George Church (Addgene plasmid # 192893 ; http://n2t.net/addgene:192893 ; RRID:Addgene_192893)

  • For your References section:

    Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression. Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM. Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291. 10.7554/eLife.83291 PubMed 36803359
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