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pHDR-FOXL2-T2A-tdTomato-PuroTK
(Plasmid #192892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHDR-MC1-DTA
  • Backbone size w/o insert (bp) 3021
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin ; Thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXL2-tdTomato
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6056
  • Mutation
    T2A-tdTomato fluorescent reporter
  • Entrez Gene
    FOXL2 (a.k.a. BPES, BPES1, PFRK, PINTO, POF3)
  • Promoter PGK

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PuroTK selection marker is flanked by Rox sites and can be excised by Dre recombinase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHDR-FOXL2-T2A-tdTomato-PuroTK was a gift from George Church (Addgene plasmid # 192892 ; http://n2t.net/addgene:192892 ; RRID:Addgene_192892)

  • For your References section:

    Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression. Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM. Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291. 10.7554/eLife.83291 PubMed 36803359
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