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E. coli BL21 (DE3) ΔEntD
(Bacterial strain #192874)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 192874 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Vector type
    This is a strain, not a plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli BL21 (DE3) ΔEntD
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    The strain has a 535 nt deletion between nt 15 and nt 551 in the EntD gene.
  • Species
    E.coli

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EntD, a 4'-phosphopantetheinyl transferase, is required for native enterobactin synthesis. This strain allows for expression of nonribosomal peptide synthetase (NRPS) carrier proteins that do not harbor a phosphopantetheinyl group.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli BL21 (DE3) ΔEntD was a gift from Christian Chalut & Christophe Guilhot (Addgene plasmid # 192874)

  • For your References section:

    The nonredundant roles of two 4'-phosphopantetheinyl transferases in vital processes of Mycobacteria. Chalut C, Botella L, de Sousa-D'Auria C, Houssin C, Guilhot C. Proc Natl Acad Sci U S A. 2006 May 30;103(22):8511-6. doi: 10.1073/pnas.0511129103. Epub 2006 May 18. 10.1073/pnas.0511129103 PubMed 16709676
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