pT2/CMV-Luc/PGK-SB100X
(Plasmid
#192869)
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PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepT2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameluc
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Alt namefirefly luciferase
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Insert Size (bp)1653
Gene/Insert 2
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Gene/Insert nameSB100X
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Alt nameSleeping Beauty transposase
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Insert Size (bp)1023
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2/CMV-Luc/PGK-SB100X was a gift from Maria Castro & David Largaespada (Addgene plasmid # 192869 ; http://n2t.net/addgene:192869 ; RRID:Addgene_192869) -
For your References section:
Transposon mediated integration of plasmid DNA into the subventricular zone of neonatal mice to generate novel models of glioblastoma. Calinescu AA, Nunez FJ, Koschmann C, Kolb BL, Lowenstein PR, Castro MG. J Vis Exp. 2015 Feb 22;(96). doi: 10.3791/52443. 10.3791/52443 PubMed 25741859
Map uploaded by the depositor.
