pMUT2-VtrA-mCherry
(Plasmid
#192862)
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PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMUT2
- Backbone size w/o insert (bp) 5601
- Total vector size (bp) 7519
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
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SpeciesVibrio cholerae
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Insert Size (bp)1918
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMUT2-VtrA-mCherry was a gift from Morten Sommer (Addgene plasmid # 192862 ; http://n2t.net/addgene:192862 ; RRID:Addgene_192862) -
For your References section:
Characterization of Eight Bacterial Biosensors for Microbial Diagnostic and Therapeutic Applications. Vaaben TH, Vazquez-Uribe R, Sommer MOA. ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 10.1021/acssynbio.2c00491. Epub 2022 Nov 30. 10.1021/acssynbio.2c00491 PubMed 36449712
Map uploaded by the depositor.
