pcDNA3-S33Y Beta-catenin
(Plasmid #19286)

• Purpose: Mammalian expression of human beta-catenin with Flag tag and S33Y mutation
• Depositing Lab: Eric Fearon
• Publication: Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin
• Alt name: CTNNB1
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Mutation: S33Y (presumptive GSK3β phosphorylation site)
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 44 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Has 12-bp sequence upstream of the initiating ATG codon matching the consensus Kozak initiator sequence.