pGEMT-mMyh3CDS
(Plasmid
#192843)
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PurposePlasmid to produce ISH probe for mMyh3 gene with CDS sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-Teasy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3016
- Total vector size (bp) 4074
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyosin heavy chain 3
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Alt nameMyh3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1058
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GenBank IDNM_001099635
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Entrez GeneMyh3 (a.k.a. MyHC-emb, Myhs-e, Myhse)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEMT-mMyh3CDS was a gift from Masafumi Inui (Addgene plasmid # 192843 ; http://n2t.net/addgene:192843 ; RRID:Addgene_192843) -
For your References section:
Digoxigenin-labeled RNA probes for untranslated regions enable the isoform-specific gene expression analysis of myosin heavy chains in whole-mount in situ hybridization. Tanji M, Wada K, Sakamoto K, Ono Y, Inui M. Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832. 10.1111/dgd.12832 PubMed 36517457