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Addgene

pGEMT-mMyh2UTR
(Plasmid #192842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-Teasy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3016
  • Total vector size (bp) 3141

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myosin heavy chain 2
  • Alt name
    Myh2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    125
  • GenBank ID
    NM_00139545
  • Entrez Gene
    Myh2 (a.k.a. MHC2A, MyHC-IIa, Myh2a, Myhs-f, Myhs-f1, Myhsf1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-mMyh2UTR was a gift from Masafumi Inui (Addgene plasmid # 192842 ; http://n2t.net/addgene:192842 ; RRID:Addgene_192842)

  • For your References section:

    Digoxigenin-labeled RNA probes for untranslated regions enable the isoform-specific gene expression analysis of myosin heavy chains in whole-mount in situ hybridization. Tanji M, Wada K, Sakamoto K, Ono Y, Inui M. Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832. 10.1111/dgd.12832 PubMed 36517457
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