pET29-hMIGA2(306-593)-6xhis
(Plasmid #192837)

• Purpose: Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria
• Depositing Lab: Karin Reinisch
• Publication: Hong et al J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 10.1083/jcb.202207022. Epub 2022 Oct 25.

Backbone

• Vector backbone: pET29a(+)
• Total vector size (bp): 6105
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon-optimized human Mitoguardin 2
• Alt name: MIGA2 (C9orf54, FAM73B)
• Species: H. sapiens (human)
• Insert Size (bp): 864
• Entrez Gene: MIGA2 (a.k.a. C9orf54, FAM73B)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xhis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tttaagaaggagatatacatatgGGCGACTATCCGATTCCGCTGAGC
• 3′ sequencing primer: tggtggtggtggtgctcgagTTGCAGCTCGCCCAGCGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry