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pCMV10-3xFLAG-prs-hMIGA2 (170-593)
(Plasmid #192833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV10
  • Total vector size (bp) 7555
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized human Mitoguardin 2
  • Alt name
    MIGA2 (C9orf54, FAM73B)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1368
  • Entrez Gene
    MIGA2 (a.k.a. C9orf54, FAM73B)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG-PreScission cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccaggggccccttgcggccgccGATGGCAACGCGGAAAGC
  • 3′ sequencing primer agggatgccacccgggatccTTATTGCAGCTCGCCCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV10-3xFLAG-prs-hMIGA2 (170-593) was a gift from Karin Reinisch (Addgene plasmid # 192833 ; http://n2t.net/addgene:192833 ; RRID:Addgene_192833)

  • For your References section:

    Mitoguardin-2-mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 10.1083/jcb.202207022. Epub 2022 Oct 25. 10.1083/jcb.202207022 PubMed 36282247
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