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pET28-6xhis-sumo-CeMIGA(106-496)
(Plasmid #192832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mitoguardin homologue
  • Alt name
    miga
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1176
  • Entrez Gene
    miga-1 (a.k.a. CELE_K01D12.6)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xhis sumo (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cagagaacagattggtggatccAGTATGAATCCAATTGAAGCAG
  • 3′ sequencing primer gtggtgctcgagtgcggccgcTTATAGTTCGTTGCTCATTCTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-6xhis-sumo-CeMIGA(106-496) was a gift from Karin Reinisch (Addgene plasmid # 192832 ; http://n2t.net/addgene:192832 ; RRID:Addgene_192832)

  • For your References section:

    Mitoguardin-2-mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 10.1083/jcb.202207022. Epub 2022 Oct 25. 10.1083/jcb.202207022 PubMed 36282247
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