pBS-GBX2-CDX124-Ef1a-mCherry-Neo
(Plasmid
#192823)
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PurposeExpresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
- Total vector size (bp) 7766
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Vector typeMammalian Expression, Cre/Lox, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsplasmid is also kanamycin resistant
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Copy numberHigh Copy
Gene/Insert
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CDX1 gRNA sequence: CTACACCGACCACCAACGCC
CDX2 gRNA sequence: GTACACGGACCACCAGCGGC
CDX4 gRNA sequence: CCTGGGCCTTTCCGAGAGAC
GBX2 gRNA sequence: AAAGGTGGAAGACGACCCGA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-GBX2-CDX124-Ef1a-mCherry-Neo was a gift from Mark Denham (Addgene plasmid # 192823 ; http://n2t.net/addgene:192823 ; RRID:Addgene_192823) -
For your References section:
Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M. Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0. 10.1038/s41467-023-43471-0 PubMed 38052784