pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
(Plasmid
#192822)
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PurposeGolden Gate compatible plasmid with mCherry and Neo
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 6724
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418) ; Kanamycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsplasmid is also Kanamycin resistant
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry and Neo
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP was a gift from Mark Denham (Addgene plasmid # 192822 ; http://n2t.net/addgene:192822 ; RRID:Addgene_192822) -
For your References section:
Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M. Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0. 10.1038/s41467-023-43471-0 PubMed 38052784