pcDNA3 WT MYOC eGLuc2 FLAG
(Plasmid #192820)

• Purpose: Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag
• Depositing Lab: John Hulleman
• Publication: Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. doi: 10.1080/02713683.2022.2047205. Epub 2022 Mar 25.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 5542
• Total vector size (bp): 7636
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myocilin
• Alt name: MYOC
• Species: H. sapiens (human)
• Insert Size (bp): 2094
• GenBank ID: 4653
• Entrez Gene: MYOC (a.k.a. GLC1A, GPOA, JOAG, JOAG1, TIGR)
• Promoter: CMV
• Tags / Fusion Proteins:
  • enhanced Gaussia luciferase (M43I/M110I) (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV For

Resource Information

• A portion of this plasmid was derived from a plasmid made by: original wild type MYOC FLAG plasmid was provided by Dr. Val Sheffield (University of Iowa).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry