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pAAV-CAG-BR1-P2A-FusionRed
(Plasmid #192819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG
  • Backbone size w/o insert (bp) 5515
  • Total vector size (bp) 5848
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BR1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    333
  • Entrez Gene
    BRI1 (a.k.a. AT4G39400, ATBRI1, BIN1, BR INSENSITIVE 1, BRASSINOSTEROID INSENSITIVE 1, CABBAGE 2, CBB2, DWARF 2, DWF2, F23K16.30, F23K16_30)
  • Promoter chicken β-actin promoter
  • Tag / Fusion Protein
    • FusionRed (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer ctccgggctgtaattagcgcttggt
  • 3′ sequencing primer catgtgaagccctcagggaaggact
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-BR1-P2A-FusionRed was a gift from Kiryl Piatkevich (Addgene plasmid # 192819 ; http://n2t.net/addgene:192819 ; RRID:Addgene_192819)

  • For your References section:

    Enhanced small green fluorescent proteins as a multisensing platform for biosensor development. Liang GT, Lai C, Yue Z, Zhang H, Li D, Chen Z, Lu X, Tao L, Subach FV, Piatkevich KD. Front Bioeng Biotechnol. 2022 Oct 17;10:1039317. doi: 10.3389/fbioe.2022.1039317. eCollection 2022. 10.3389/fbioe.2022.1039317 PubMed 36324888
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