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pMRX-IP-3xFLAG-NCOA4 (L63R)
(Plasmid #192814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NCOA4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1842
  • Mutation
    L63R mutation in the N-terminal domain
  • Entrez Gene
    NCOA4 (a.k.a. ARA70, ELE1, PTC3, RFG)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IP-3xFLAG-NCOA4 (L63R) was a gift from Noboru Mizushima (Addgene plasmid # 192814 ; http://n2t.net/addgene:192814 ; RRID:Addgene_192814)

  • For your References section:

    NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and microferritinophagy. Ohshima T, Yamamoto H, Sakamaki Y, Saito C, Mizushima N. J Cell Biol. 2022 Oct 3;221(10). pii: 213442. doi: 10.1083/jcb.202203102. Epub 2022 Sep 6. 10.1083/jcb.202203102 PubMed 36066504
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