pMRX-IB-mRuby3-FTH1
(Plasmid
#192804)
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PurposeStable expression of mRuby3-FTH1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192804 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRX-IB
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFTH1
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SpeciesH. sapiens (human)
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Insert Size (bp)549
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Entrez GeneFTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
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Tag
/ Fusion Protein
- mRuby3 (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IB-mRuby3-FTH1 was a gift from Noboru Mizushima (Addgene plasmid # 192804 ; http://n2t.net/addgene:192804 ; RRID:Addgene_192804) -
For your References section:
NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and microferritinophagy. Ohshima T, Yamamoto H, Sakamaki Y, Saito C, Mizushima N. J Cell Biol. 2022 Oct 3;221(10). pii: 213442. doi: 10.1083/jcb.202203102. Epub 2022 Sep 6. 10.1083/jcb.202203102 PubMed 36066504