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Addgene

pFastBac dRING1F
(Plasmid #1928)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dRING1
  • Alt name
    Sce
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Sce (a.k.a. Dmel_CG5595, CG5595, DRING, Ding, Dmel\CG5595, Dring, RING, RING1, Ring, Ring/Sce, Rnf2, SCE, Sce/dRing, dRING, dRING1, dRing, dRing1, dring, sce)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For baculoviral expression of dRING1F with C-terminal flag epitope. 300bp of C-terminus of pFB-dRING1 was amplified with SacI--FLAG-KpnI primers and cloned into dRING1 with SacI--KpnI to produce dRING1 with C-terminal FLAG epitope (kingston #1325)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac dRING1F was a gift from Robert Kingston (Addgene plasmid # 1928 ; http://n2t.net/addgene:1928 ; RRID:Addgene_1928)

  • For your References section:

    Reconstitution of a functional core polycomb repressive complex. Francis NJ, Saurin AJ, Shao Z, Kingston RE. Mol Cell 2001 Sep;8(3):545-56. 10.1016/S1097-2765(01)00316-1 PubMed 11583617
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