Ppst93-MCRaeo-NC
(Plasmid
#192770)
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PurposeExpresses NC-tagged MCRaeo in Methanococcus maripaludis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192770 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepMEV5
- Backbone size w/o insert (bp) 4300
- Total vector size (bp) 9500
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Vector typeSynthetic Biology ; Heterologous expression in Methanococcus maripaludis
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMethyl coenzyme M reductase
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Alt nameMCR
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SpeciesMethanococcus aeolicus
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Insert Size (bp)5200
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Tag
/ Fusion Protein
- Flag-Strep2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ppst93-MCRaeo-NC was a gift from William B. Whitman (Addgene plasmid # 192770 ; http://n2t.net/addgene:192770 ; RRID:Addgene_192770) -
For your References section:
Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea. Shao N, Fan Y, Chou CW, Yavari S, Williams RV, Amster IJ, Brown SM, Drake IJ, Duin EC, Whitman WB, Liu Y. Commun Biol. 2022 Oct 20;5(1):1113. doi: 10.1038/s42003-022-04057-6. 10.1038/s42003-022-04057-6 PubMed 36266535