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Addgene

Ppst93-MCRaeo-NG mcrBGA
(Plasmid #192769)

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Item Catalog # Description Quantity Price (USD)
Plasmid 192769 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMEV5
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 8300
  • Vector type
    Synthetic Biology ; Heterologous expression in Methanococcus maripaludis
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methyl coenzyme M reductase
  • Alt name
    MCR
  • Species
    Methanococcus aeolicus
  • Insert Size (bp)
    4000
  • Tag / Fusion Protein
    • Flag-Strep2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ppst93-MCRaeo-NG mcrBGA was a gift from William B. Whitman (Addgene plasmid # 192769 ; http://n2t.net/addgene:192769 ; RRID:Addgene_192769)

  • For your References section:

    Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea. Shao N, Fan Y, Chou CW, Yavari S, Williams RV, Amster IJ, Brown SM, Drake IJ, Duin EC, Whitman WB, Liu Y. Commun Biol. 2022 Oct 20;5(1):1113. doi: 10.1038/s42003-022-04057-6. 10.1038/s42003-022-04057-6 PubMed 36266535
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