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Addgene

pDONR221-appa
(Plasmid #192745)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192745 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    appa
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2049
  • Mutation
    S47N
  • Entrez Gene
    appa (a.k.a. app, im:704454, im:7044540, wu:fj08a1, wu:fj08a12, wu:fj34d10, wu:fk65e12, zgc:85740)
  • Promoter None

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA encodes AFN73054.1; homolog of human APP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-appa was a gift from Summer Thyme (Addgene plasmid # 192745 ; http://n2t.net/addgene:192745 ; RRID:Addgene_192745)