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Addgene

pDONR221-brwd1
(Plasmid #192742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    brwd1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    6549
  • Mutation
    K144W; D145E; I760T (rs512641576); Y770F (rs507445530); T772I (rs500259283); D896E (rs504506526); H921Q (rs510496334); A924S (rs514461947); D1147V; Q1308E; A1490V; M1531R; H1579Q; R1602K; E1657D; A1660E; R1785S; L1914P
  • Entrez Gene
    brwd1 (a.k.a. im:7140700)
  • Promoter None

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA encodes XP_001920441.4; homolog of human BRWD1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-brwd1 was a gift from Summer Thyme (Addgene plasmid # 192742 ; http://n2t.net/addgene:192742 ; RRID:Addgene_192742)
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