pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
(Plasmid
#192714)
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PurposeMammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSDest
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Backbone manufacturerAddgene #22423
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCP-ADAR2-DD(E488Q/T490A)-V5
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SpeciesH. sapiens (human)
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Insert Size (bp)1986
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MutationE488Q/T490A
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthesized by Twist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5 was a gift from Summer Thyme (Addgene plasmid # 192714 ; http://n2t.net/addgene:192714 ; RRID:Addgene_192714)
