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Addgene

pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
(Plasmid #192713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist ENTR Kozak
  • Backbone manufacturer
    Twist Bioscience
  • Vector type
    Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCP-ADAR2-DD(E488Q/T490A)-V5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1986
  • Mutation
    E488Q/T490A
  • Promoter None
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5 was a gift from Summer Thyme (Addgene plasmid # 192713 ; http://n2t.net/addgene:192713 ; RRID:Addgene_192713)
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