pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
(Plasmid
#192705)
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PurposePlasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBDest
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Backbone manufacturerVienna Biocenter Core Facilities GmbH, Vienna, Austria
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 13584
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameATG5
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Alt nameAPG5
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SpeciesH. sapiens (human)
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Insert Size (bp)894
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GenBank IDNC_000006.12
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Tag
/ Fusion Protein
- 10x-His-TEV (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer ATGCACCATCACCATCACCATCACCATCACCATG
- 3′ sequencing primer CCCCAGAACATCAGGTTAATGGCGTTAGTCCGTAGGTTGG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameATG7
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SpeciesH. sapiens (human)
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Insert Size (bp)2112
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GenBank IDNC_000003.12
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer ATGGCAGCGGCTACTGGTGACCCTGGCTTG
- 3′ sequencing primer TTAAATAGTCTCATCGTCGCTCATATCCCA (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameATG10
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SpeciesH. sapiens (human)
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Insert Size (bp)663
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GenBank IDNC_000005.10
Cloning Information for Gene/Insert 3
- Cloning method Gateway Cloning
- 5′ sequencing primer ATGGAGGAGGACGAGTTCATCGGAGAAAAG
- 3′ sequencing primer TTAGGGCACGTTTCTTTCGTCTTGGC (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameATG12
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SpeciesH. sapiens (human)
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Insert Size (bp)423
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GenBank IDNC_000005.10
Cloning Information for Gene/Insert 4
- Cloning method Gateway Cloning
- 5′ sequencing primer ATGGCCGAAGAGCCCCAGAGCGTTT
- 3′ sequencing primer TTAGCCCCACGCTTGTGATTTGCAGTAATG (Common Sequencing Primers)
Gene/Insert 5
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Gene/Insert nameATG16L1
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Insert Size (bp)2631
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MutationF32A-I35A-I36A
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GenBank IDNC_000002.12
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Tag
/ Fusion Protein
- -GFP-TEV-StrepII (C terminal on insert)
Cloning Information for Gene/Insert 5
- Cloning method Gateway Cloning
- 5′ sequencing primer ATGAGCAGCGGTTTGAGGGCGGCGGATTTC
- 3′ sequencing primer TTACTTCTCGAACTGCGGATGCGACCAGGCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII was a gift from Sascha Martens (Addgene plasmid # 192705 ; http://n2t.net/addgene:192705 ; RRID:Addgene_192705)