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pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
(Plasmid #192705)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192705 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBDest
  • Backbone manufacturer
    Vienna Biocenter Core Facilities GmbH, Vienna, Austria
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 13584
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATG5
  • Alt name
    APG5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    894
  • GenBank ID
    NC_000006.12
  • Entrez Gene
    ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
  • Tag / Fusion Protein
    • 10x-His-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGCACCATCACCATCACCATCACCATCACCATG
  • 3′ sequencing primer CCCCAGAACATCAGGTTAATGGCGTTAGTCCGTAGGTTGG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATG7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2112
  • GenBank ID
    NC_000003.12
  • Entrez Gene
    ATG7 (a.k.a. APG7-LIKE, APG7L, GSA7, SCAR31)

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGCAGCGGCTACTGGTGACCCTGGCTTG
  • 3′ sequencing primer TTAAATAGTCTCATCGTCGCTCATATCCCA
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    ATG10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    663
  • GenBank ID
    NC_000005.10
  • Entrez Gene
    ATG10 (a.k.a. APG10, APG10L, pp12616)

Cloning Information for Gene/Insert 3

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGAGGAGGACGAGTTCATCGGAGAAAAG
  • 3′ sequencing primer TTAGGGCACGTTTCTTTCGTCTTGGC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    ATG12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • GenBank ID
    NC_000005.10
  • Entrez Gene
    ATG12 (a.k.a. APG12, APG12L, FBR93, HAPG12)

Cloning Information for Gene/Insert 4

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGCCGAAGAGCCCCAGAGCGTTT
  • 3′ sequencing primer TTAGCCCCACGCTTGTGATTTGCAGTAATG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    ATG16L1
  • Insert Size (bp)
    2631
  • Mutation
    F32A-I35A-I36A
  • GenBank ID
    NC_000002.12
  • Tag / Fusion Protein
    • -GFP-TEV-StrepII (C terminal on insert)

Cloning Information for Gene/Insert 5

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGAGCAGCGGTTTGAGGGCGGCGGATTTC
  • 3′ sequencing primer TTACTTCTCGAACTGCGGATGCGACCAGGCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII was a gift from Sascha Martens (Addgene plasmid # 192705 ; http://n2t.net/addgene:192705 ; RRID:Addgene_192705)
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