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Addgene

pCR-U6-gRNA-miniCMV-Nluc
(Plasmid #192657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nluc
  • gRNA/shRNA sequence
    miniCMV sgRNA: GTCCCCTCCACCCCACAGTG
  • Species
    Synthetic
  • Promoter U6, miniCMV

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-U6-gRNA-miniCMV-Nluc was a gift from Jeffrey Miner (Addgene plasmid # 192657 ; http://n2t.net/addgene:192657 ; RRID:Addgene_192657)

  • For your References section:

    Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activation. Omachi K, Miner JH. PLoS One. 2022 Jun 28;17(6):e0270008. doi: 10.1371/journal.pone.0270008. eCollection 2022. 10.1371/journal.pone.0270008 PubMed 35763517
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