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Addgene

pCK760
(Plasmid #192643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJF143.J3 (Addgene #153028)
  • Backbone manufacturer
    Jesse Zalatan
  • Backbone size w/o insert (bp) 3368
  • Total vector size (bp) 5546
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BBa_J23110-sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1108
  • Promoter BBa_J23110

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTAGAAAAATAAACAAATAGGGGTTCCGCGC
  • 3′ sequencing primer ACAGGATGTCCCAAGCGAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is low copy and therefore has a higher percentage of E. coli genomic DNA. To get high concentration / high purity plasmid of pSC101 backbone, we usually grow multiple cultures and pool them into one column for miniprep. For Qiagen miniprep kit, we included PB wash step to increase the purity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK760 was a gift from Jesse Zalatan (Addgene plasmid # 192643 ; http://n2t.net/addgene:192643 ; RRID:Addgene_192643)

  • For your References section:

    Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants. Kiattisewee C, Karanjia AV, Legut M, Daniloski Z, Koplik SE, Nelson J, Kleinstiver BP, Sanjana NE, Carothers JM, Zalatan JG. ACS Synth Biol. 2022 Nov 15. doi: 10.1021/acssynbio.2c00405. 10.1021/acssynbio.2c00405 PubMed 36378874
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