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pET 28 full-length human PARP1
(Plasmid #192635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human PARP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3042
  • Mutation
    Polymorphism A762
  • Entrez Gene
    PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
  • Tag / Fusion Protein
    • histidine-tag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET 28 full-length human PARP1 was a gift from John Pascal (Addgene plasmid # 192635 ; http://n2t.net/addgene:192635 ; RRID:Addgene_192635)

  • For your References section:

    A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordinates DNA-dependent enzyme activation. Langelier MF, Servent KM, Rogers EE, Pascal JM. J Biol Chem. 2008 Feb 15;283(7):4105-14. doi: 10.1074/jbc.M708558200. Epub 2007 Nov 30. 10.1074/jbc.M708558200 PubMed 18055453
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