AAV.pCry1(408).DIO.Luciferase
(Plasmid
#192623)
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PurposeCre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 4053
- Total vector size (bp) 6385
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameluciferase
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SpeciesFirefly
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Insert Size (bp)1653
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV.pCry1(408).DIO.Luciferase was a gift from Michael Hastings (Addgene plasmid # 192623 ; http://n2t.net/addgene:192623 ; RRID:Addgene_192623) -
For your References section:
Cell-autonomous clock of astrocytes drives circadian behavior in mammals. Brancaccio M, Edwards MD, Patton AP, Smyllie NJ, Chesham JE, Maywood ES, Hastings MH. Science. 2019 Jan 11;363(6423):187-192. doi: 10.1126/science.aat4104. 10.1126/science.aat4104 PubMed 30630934