pAAV-CAG-RiboL1-jGCaMP8m
(Plasmid #192613)

• Purpose: AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.
• Depositing Lab: Marianne Fyhn
• Publication: Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4742
• Total vector size (bp): 6740
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RiboL1-jGCaMP8m
• Insert Size (bp): 1998
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Resource Information

• Supplemental Documents:
  • paav-cag-ribol1-jgcamp8m-wpre-sv40polya.gb
• A portion of this plasmid was derived from a plasmid made by: jGCaMP8m from plasmid #162375.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CAG backbone plasmid: #59462.