CaMKII-somBiPOLES-eGFP
(Plasmid
#192583)
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PurposeAAV-vector for bidirectional optogenetic manipulation of excitatory neurons
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
- Backbone size w/o insert (bp) 3776
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesomBiPOLES
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SpeciesSynthetic; Guillardia theta; Chlamydomonas noctigama
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Insert Size (bp)3279
- Promoter CaMKIIa(0.4)
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Tag
/ Fusion Protein
- soma-targeting motif from Kv2.1 channel (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The insert contains the fluorescence tag EGFP in between the 2 channelrhodopsins (C-terminal of GtACR2)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaMKII-somBiPOLES-eGFP was a gift from Simon Wiegert (Addgene plasmid # 192583 ; http://n2t.net/addgene:192583 ; RRID:Addgene_192583)