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TRE3GV-somBiPOLES-mCerulean
(Plasmid #192578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone size w/o insert (bp) 4261
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    somBiPOLES
  • Species
    Synthetic; Guillardia theta; Chlamydomonas noctigama
  • Insert Size (bp)
    3276
  • Promoter TRE3GV
  • Tag / Fusion Protein
    • soma-targeting motif from Kv2.1 channel (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert contains the fluorescence tag mCerulean3 in between the 2 channelrhodopsins (C-terminal of GtACR2)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE3GV-somBiPOLES-mCerulean was a gift from Simon Wiegert (Addgene plasmid # 192578 ; http://n2t.net/addgene:192578 ; RRID:Addgene_192578)
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