Ef1a-DIO-somAion-citrine
(Plasmid
#192573)
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PurposeAAV-vector for optogenetic long-term silencing of Cre-positive neurons
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
- Backbone size w/o insert (bp) 5616
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesomAion
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SpeciesSynthetic; Chlamydomonas reinhardtii
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Insert Size (bp)1854
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MutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156C, T159G, G163A, V242R, T246N, N258Q, E273S
- Promoter Ef1-alpha
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Tag
/ Fusion Protein
- Citrine - soma-targeting motif from Kv2.1 channel (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unspecified (destroyed during cloning)
- 3′ cloning site unspecified (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ef1a-DIO-somAion-citrine was a gift from Simon Wiegert (Addgene plasmid # 192573 ; http://n2t.net/addgene:192573 ; RRID:Addgene_192573)