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Addgene

hSyn-somAion-citrine
(Plasmid #192570)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192570 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone size w/o insert (bp) 4369
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    somAion
  • Species
    Synthetic; Chlamydomonas reinhardtii
  • Insert Size (bp)
    1854
  • Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156C, T159G, G163A, V242R, T246N, N258Q, E273S
  • Promoter human synapsin
  • Tag / Fusion Protein
    • Citrine - soma-targeting motif from Kv2.1 channel (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn-somAion-citrine was a gift from Simon Wiegert (Addgene plasmid # 192570 ; http://n2t.net/addgene:192570 ; RRID:Addgene_192570)

  • For your References section:

    Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing. Rodriguez-Rozada S, Wietek J, Tenedini F, Sauter K, Dhiman N, Hegemann P, Soba P, Wiegert JS. Commun Biol. 2022 Jul 9;5(1):687. doi: 10.1038/s42003-022-03636-x. 10.1038/s42003-022-03636-x PubMed 35810216
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